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This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field.
ISBN: 9781071614983
Auflage: 2
Sprache: Englisch
Seitenzahl: 288
Produktart: Gebunden
Herausgeber: Ponchon, Luc
Verlag: Springer US
Veröffentlicht: 05.06.2021
Untertitel: Methods and Protocols
Schlagworte: Aptazyme-Based Riboswitches Hammerhead Ribozymes In-Cell SHAPE RNA-protein interaction RNA Mango aptamers

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