RNA Abundance Analysis
This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.
ISBN: | 9781071607428 |
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Auflage: | 2 |
Sprache: | Englisch |
Seitenzahl: | 233 |
Produktart: | Gebunden |
Herausgeber: | Jin, Hailing Kaloshian, Isgouhi |
Verlag: | Springer US |
Veröffentlicht: | 15.08.2020 |
Untertitel: | Methods and Protocols |
Schlagworte: | Gene expression Next-generation sequencing RNA-protein interactions RNA expression Small RNAs mRNAs |