Zum Hauptinhalt springen Zur Suche springen Zur Hauptnavigation springen
Herzlich Willkommen!
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
Autor: Köhler, Thomas Laßner, Dirk Pustowoit, Barbara Remke, Harald Rost, Anne-Katrin Thamm, Barbara
ISBN: 9783642797149
Sprache: Englisch
Seitenzahl: 166
Produktart: Kartoniert / Broschiert
Verlag: Springer Berlin
Veröffentlicht: 29.12.2011
Untertitel: Nonradioactive PCR Methods
Schlagworte: Analyse DNA DNA sequencing Nucleotide PCR Quantitative PCR RNA chemical analysis chemische Analyse enzymes