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New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct the appropriate procedures which fulfill their particular requirements.
ISBN: 9783540646013
Auflage: 2
Sprache: Englisch
Seitenzahl: 750
Produktart: Kartoniert / Broschiert
Herausgeber: Kessler, Christoph
Verlag: Springer Berlin
Veröffentlicht: 11.03.2000
Schlagworte: Activation Chemolumineszenz DNA DNA-Nachweis In Situ Hybridisierung Luminescence Nucleotide PCR Proteinnachweismethoden RNA