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This volume covers the latest experimental protocols used by researchers to study the recently emerged fungal pathogen of humans, Candida auris , and also explores the pathogen’s antifungal resistance, virulence, and pathogenicity. The chapters in this book cover topics such as the identification of C. auris in clinic samples by PCR and mass spectrometry; molecular typing to identify clade affiliation; genetic manipulation; genome analysis by bioinformatics; and phenotypic characterization using various biochemical, mass spectrometry, microscopy, and flow cytometry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Candida auris: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field.
ISBN: 9781071624166
Sprache: Englisch
Seitenzahl: 342
Produktart: Gebunden
Herausgeber: Lorenz, Alexander
Verlag: Springer US
Veröffentlicht: 09.06.2022
Untertitel: Methods and Protocols
Schlagworte: Cell viability assays Echinocandins Fungal Predators Hsp90 MALDI-TOF MS

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